UQCRFS1 Antibody

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex (By similarity) .

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25

UniProt

P47985

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 181-209 from the human protein was used as the immunogen for the UQCRFS1 antibody.

Clonality

Polyclonal

Isotype

Applications

IF, FACS, WB, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the UQCRFS1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This UQCRFS1 antibody is available for research use only.

Storage Conditions

Aliquot the UQCRFS1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the UQCRFS1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human rectum tissue with UQCRFS1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.