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Sciellin Antibody / SCEL

The protein encoded by this gene is a precursor to the cornified envelope of terminally differentiated keratinocytes. This protein localizes to the periphery of cells and may function in the assembly or regulation of proteins in the cornified envelope. Transcript variants encoding different isoforms exist. A transcript variant utilizing an alternative polyA signal has been described in the literature, but its full-length nature has not been determined.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells)

UniProt

O95171

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 260-289 from the human protein was used as the immunogen for the Sciellin antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

IHC-P, WB, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Sciellin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Sciellin antibody is available for research use only.

Storage Conditions

Aliquot the Sciellin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.nsjbio.com/tds-pdf/sciellin-antibody-scel-f54353

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Sciellin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, plasma membrane

Image Legend

IHC testing of FFPE human skin tissue with Sciellin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.

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