Complement factor H Antibody / CFH

This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short concensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000, Immunofluorescence: 1:25

UniProt

P08603

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 751-780 from the human protein was used as the immunogen for the Complement factor H antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P, WB, IF

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Complement factor H antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Complement factor H antibody is available for research use only.

Storage Conditions

Aliquot the Complement factor H antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Complement factor H antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human 1) lung and 2) plasma lysate with Complement factor H antibody. Predicted molecular weight ~139 kDa but may be observed at a higher molecular weight due to glycosylation.