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COL5A1 Antibody

COL5A1 encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. COL5A1 product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. [provided by RefSeq].

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

P20908

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 54-82 from the human protein was used as the immunogen for the COL5A1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the COL5A1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This COL5A1 antibody is available for research use only.

Storage Conditions

Aliquot the COL5A1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the COL5A1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC testing of FFPE human uterus tissue with COL5A1 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.

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