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CHRM2 Antibody

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells)

UniProt

P08172

Host

Mouse

Reactivity

Human, Mouse

Immunogen

A recombinant human protein was used as the immunogen for the CHRM2 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

1424CT461.78.60

Applications

IHC-P, WB, IF, FACS

Purity

Protein G affinity

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the CHRM2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CHRM2 antibody is available for research use only.

Storage Conditions

Aliquot the CHRM2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the CHRM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC testing of FFPE human brain tissue with CHRM2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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