PPARA Antibody / PPAR alpha

Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC) . Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity) . Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000

UniProt

Q07869

Host

Mouse

Reactivity

Human, Mouse

Immunogen

A recombinant human protein was used as the immunogen for the PPARA antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

1331CT894.186.143

Applications

IHC-P, IF, FACS, WB

Purity

Protein G affinity

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PPARA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PPARA antibody is available for research use only.

Storage Conditions

Aliquot the PPARA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PPARA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear

Image Legend

IHC testing of FFPE human skeletal muscle tissue with PPARA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.