PPT1 Antibody

Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000

UniProt

P50897

Host

Mouse

Reactivity

Human

Immunogen

Full length recombinant human protein was used as the immunogen for the PPT1 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

1117CT11.2.1.4

Applications

IHC-P, FACS, WB

Purity

Protein G affinity

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PPT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PPT1 antibody is available for research use only.

Storage Conditions

Aliquot the PPT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PPT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human cerebellum tissue with PPT1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.