Sclerostin Antibody / SOST

Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

Q9BQB4

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A portion of amino acids 12-42 from the human protein was used as the immunogen for the Sclerostin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Sclerostin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Sclerostin antibody is available for research use only.

Storage Conditions

Aliquot the Sclerostin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Sclerostin antibody may be required due to differences in protocols and secondary/substrate sensitivity.