Autotaxin Antibody / ENPP2

ENPP2 functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This protein stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The protein is secreted and further processed to make the biologically active form.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells)

UniProt

Q13822

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 377-406 from the human protein was used as the immunogen for the Autotaxin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Autotaxin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Autotaxin antibody is available for research use only.

Storage Conditions

Aliquot the Autotaxin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Autotaxin antibody may be required due to differences in protocols and secondary/substrate sensitivity.