Hsp60 Antibody

Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25

UniProt

P10809

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 340-374 from the human protein was used as the immunogen for the Hsp60 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, IF

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Hsp60 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Hsp60 antibody is available for research use only.

Storage Conditions

Aliquot the Hsp60 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Hsp60 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of human 1) HeLa, 2) MCF7, 3) HepG2, 4) Jurkat, 5) mouse NIH 3T3 and 6) mouse thymus lysate with Hsp60 antibody. Predicted molecular weight: ~60 kDa.