Thymidylate Synthase Antibody / TYSY
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells)
UniProt
P04818
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 265-294 from the human protein was used as the immunogen for the Thymidylate Synthase Antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Thymidylate Synthase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Thymidylate Synthase antibody is available for research use only.
Storage Conditions
Aliquot the Thymidylate Synthase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Location
Cytoplasmic, nuclear
Image Legend
Western blot testing of human 1) HeLa, 2) Jurkat and 3) HL60 cell lysate with Thymidylate Synthase antibody. Predicted molecular weight ~36 kDa.
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