Hexosaminidase A Antibody / HEXA

HEXA is the alpha subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Flow cytometry: 1:25 (1x10e6 cells)

UniProt

P06865

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 142-170 from the human protein were used as the immunogen for the Hexosaminidase A antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Hexosaminidase A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Hexosaminidase A antibody is available for research use only.

Storage Conditions

Aliquot the Hexosaminidase A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Hexosaminidase A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human liver tissue with Hexosaminidase A antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.