VIL1 Antibody / Villin 1

Villin-1 is a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Flow cytometry: 1:25 (1x10e6 cells)

UniProt

P09327

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 180-207 from the human protein were used as the immunogen for the VIL1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the VIL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This VIL1 antibody is available for research use only.

Storage Conditions

Aliquot the VIL1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the VIL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic and cell surface

Image Legend

Western blot testing of 1) human HT-29, 2) human HepG2, 3) human COLO205 and 4) mouse colon lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.