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Glyoxalase I Antibody / GLO1

The enzyme GLO1 is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells)

UniProt

Q04760

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 23-51 from the human protein were used as the immunogen for the Glyoxalase I antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC-P, IF, FACS

Purity

SAS precipitation

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Glyoxalase I antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Glyoxalase I antibody is available for research use only.

Storage Conditions

Aliquot the Glyoxalase I antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Glyoxalase I antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human 1) HeLa and 2) HepG2 cell lysate with Glyoxalase I antibody. Predicted molecular weight ~21 kDa.

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