AP1M1 Antibody

The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq].

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25

UniProt

Q9BXS5

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 199-227 from the human protein were used as the immunogen for the AP1M1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the AP1M1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This AP1M1 antibody is available for research use only.

Storage Conditions

Aliquot the AP1M1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the AP1M1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) human PC-3, 2) human U-251 MG, 3) mouse NIH 3T3 and 4) mouse heart lysate with AP1M1 antibody. Predicted molecular weight ~49 kDa.