HDAC2 Antibody

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25

UniProt

Q92769

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 456-488 from the human protein were used as the immunogen for the HDAC2 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, IHC-P

Purity

SAS precipitation

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the HDAC2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HDAC2 antibody is available for research use only.

Storage Conditions

Aliquot the HDAC2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the HDAC2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.