NKG2A Antibody / KLRC1

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. This protein belongs to the killer cell lectin-like receptor family, also called NKG2 family, which is a group of transmembrane proteins preferentially expressed in NK cells. This family of proteins is characterized by the type II membrane orientation and the presence of a C-type lectin domain. This protein forms a complex with another family member, KLRD1/CD94, and has been implicated in the recognition of the MHC class I HLA-E molecules in NK cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells)

UniProt

P26715

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 180-206 from the human protein were used as the immunogen for the NKG2A antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the NKG2A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This NKG2A antibody is available for research use only.

Storage Conditions

Aliquot the NKG2A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the NKG2A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human 1) K562 and 2) HepG2 cell lysate with NKG2A antibody. Predicted molecular weight: 26-40 kDa depending on glycosylation level.