HSPA5 Antibody / GRP78 / BiP
In cooperation with other chaperones, HSP70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. The HSP70s in mitochondria and the endoplasmic reticulum play an additional role by providing a driving force for protein translocation. They are involved in signal transduction pathways in cooperation with HSP90. They participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells)
UniProt
P11021
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
Full length recombinant human protein was used as the immunogen for the HSPA5 Antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC-P, IF, FACS
Purity
SAS precipitation
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the HSPA5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HSPA5 antibody is available for research use only.
Storage Conditions
Aliquot the HSPA5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Location
Cytoplasmic
Image Legend
Western blot testing of human HL60 cell lysate with HSPA5 antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.
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