HIPPI Antibody / IFT57

Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity) . Has pro- apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 1:2000

UniProt

Q9NWB7

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 159-193 from the human protein was used as the immunogen for the HIPPI antibody.

Clonality

Polyclonal

Isotype

Applications

Purity

Peptide affinity purified

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the HIPPI antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HIPPI antibody is available for research use only.

Storage Conditions

Aliquot the HIPPI antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the HIPPI antibody may be required due to differences in protocols and secondary/substrate sensitivity.