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NF-H Antibody / Neurofilament (Heavy) / NEFH (Center Region)

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000-1:2000

UniProt

P12036

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 328-362 from human NEFH was used as the immunogen for the NF-H antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the NF-H antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This NF-H antibody is available for research use only.

Storage Conditions

Aliquot the NF-H antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the NF-H antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of 1) human SH-SY5Y and 2) mouse Neuro-2a cell lysate with NF-H antibody at 1:2000. Predicted molecular weight ~112 kDa, often visualized at ~200 kDa.

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