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DNAJC3 Antibody (Center Region)

DnaJ homolog subfamily C member 3 is involved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000-1:2000

UniProt

Q13217

Host

Rabbit

Reactivity

Mouse

Immunogen

A portion of amino acids 189-223 from human DNAJC3 was used as the immunogen for the DNAJC3 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the DNAJC3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This DNAJC3 antibody is available for research use only.

Storage Conditions

Aliquot the DNAJC3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the DNAJC3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Human, Rat

Image Legend

Western blot testing of mouse NIH3T3 cell lysate with DNAJC3 antibody at 1:2000. Predicted molecular weight ~58 kDa.

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