BRAF V600E Antibody

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000-1:2000

UniProt

P15056

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 576-606 from human BRAF was used as the immunogen for the BRAF V600E antibody.

Clonality

Polyclonal

Isotype

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the BRAF V600E antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This BRAF V600E antibody is available for research use only.

Storage Conditions

Aliquot the BRAF V600E antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the BRAF V600E antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Chicken, Mouse, Rat

Image Legend

Western blot testing of 1) BRAF-600V, 2) BRAF-V600E, 3) Raji, 4) HeLa, 5) A549, 6) T47D and 7) MDA-MB-453 lysate with BRAF V600E antibody at 1:2000. Predicted molecular weight ~85-95 kDa.