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PKC alpha Antibody / aPKC

This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

P17252

Host

Mouse

Reactivity

Rat

Immunogen

A synthetic PKC alpha/aPKC peptide was used as the immunogen for the PKC alpha antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

3G11-G11-G11

Applications

WB

Purity

Protein G affinity

Format

Purified

Buffer

Antibody in PBS with 50% Glycerol and 0.03% ProClin 300

Reconstitution

Store the PKC alpha antibody at -20oC.

Limitations

This PKC alpha antibody is available for research use only.

Storage Conditions

Store the PKC alpha antibody at -20°C.

Formulation

In PBS with 50% glycerol and 0.03% ProClin 300

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PKC alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Human

Image Legend

Western blot testing of 1) human C2C12, 2) monkey COS7 and 3) rat C6 cell lysates using PKC alpha antibody at 1:1000. Predicted molecular weight ~77 kDa.

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