LC3A/B Antibody

LC3B is a Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) . Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

Q9H492, Q9GZQ8

Host

Mouse

Reactivity

Human, Rat

Immunogen

A synthetic LC3B peptide was used as the immunogen for this LC3A/B antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

3E9-E5-C9

Applications

Purity

Protein G affinity

Format

Purified

Buffer

Antibody in PBS with 50% Glycerol and 0.03% ProClin 300

Reconstitution

Store the LC3A/B antibody at -20oC.

Limitations

This LC3A/B antibody is available for research use only.

Storage Conditions

Store the LC3A/B antibody at -20°C.

Formulation

In PBS with 50% glycerol and 0.03% ProClin 300

Applications Notes

The stated application concentrations are suggested starting points. Titration of the LC3A/B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of 1) rat cerebral cortex, 2) rat brain and 3) human HeLa cell lysates using LC3A/B antibody at 1:1000. Predicted molecular weight: ~14 kDa (LC3A) and ~15 kDa (LC3B) .