ATG5 Antibody
Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. May play
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
UniProt
Q9H1Y0
Host
Mouse
Reactivity
Human, Rat
Immunogen
A synthetic protein specific to ATG5 was used as the immunogen for the ATG5 antibody.
Clonality
Monoclonal
Isotype
LgG1
Clone
8F1-2C11-D9
Applications
WB
Purity
Protein G affinity
Format
Purified
Buffer
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
Reconstitution
Store the ATG5 antibody at -20oC.
Limitations
This ATG5 antibody is available for research use only.
Storage Conditions
Store the ATG5 antibody at -20°C.
Formulation
In PBS with 50% glycerol and 0.03% ProClin 300
Applications Notes
The stated application concentrations are suggested starting points. Titration of the ATG5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of 1) huma HCT116, 2) rat brain, 3) human K562 and 4) rat C6 cell lysates using ATG5 antibody at 1:1000. Predicted molecular weight ATG5: ~32 kDa; ATG5/ATG12 heterodimer: ~56 kDa.
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