EIF4E Antibody

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

P06730

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A human recombinant protein was used as the immunogen for this EIF4E antibody.

Clonality

Monoclonal

Isotype

LgG1

Clone

8C8-C10-C12

Applications

Purity

Protein G affinity

Format

Purified

Buffer

Antibody in PBS with 50% Glycerol and 0.03% ProClin 300

Reconstitution

Store the EIF4E antibody at -20oC.

Limitations

This EIF4E antibody is available for research use only.

Storage Conditions

Store the EIF4E antibody at -20°C.

Formulation

In PBS with 50% glycerol and 0.03% ProClin 300

Applications Notes

The stated application concentrations are suggested starting points. Titration of the EIF4E antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human 1) Jurkat, 2) HeLa, 3) MCF7, 4) K562, 5) rat C6, 6) mouse NIH3T3, 7) rat PC12, 8) human HepG2, 9) hamster CHO-K1, 10) human Raji, 11) mouse Raw264.7 and 12) human Ramos cell lysates using EIF4E antibody at 1:2000. Predicted molecular weight ~27 kDa.