CDK4 Antibody

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500

UniProt

P11802

Host

Mouse

Reactivity

Human

Immunogen

A human recombinant protein was used as the immunogen for this CDK4 antibody.

Clonality

Monoclonal

Isotype

LgG1

Clone

3D12-A7-F11

Applications

Purity

Protein G affinity

Format

Purified

Buffer

Antibody in PBS with 50% Glycerol and 0.03% ProClin 300

Reconstitution

Store the CDK4 antibody at -20oC.

Limitations

This CDK4 antibody is available for research use only.

Storage Conditions

Store the CDK4 antibody at -20°C.

Formulation

In PBS with 50% glycerol and 0.03% ProClin 300

Applications Notes

The stated application concentrations are suggested starting points. Titration of the CDK4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.