Phospho-ERK1/2 Antibody (Thr202/Tyr204)

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500

UniProt

P27361, P28482

Host

Mouse

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to the amino acids surrounding pThr202 and pTyr204 of human ERK1/2 was used as the immunogen for this phospho-ERK1/2 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

2E8-2F6-C12

Applications

Purity

Protein G affinity

Format

Purified

Buffer

Antibody in PBS with 50% Glycerol and 0.03% ProClin 300

Reconstitution

Store the phospho-ERK1/2 antibody at -20oC.

Limitations

This phospho-ERK1/2 antibody is available for research use only.

Storage Conditions

Store the phospho-ERK1/2 antibody at -20°C.

Formulation

In PBS with 50% glycerol and 0.03% ProClin 300

Applications Notes

The stated application concentrations are suggested starting points. Titration of the phospho-ERK1/2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of EGF-treated and untreated A549 cells and PDGF-treated and untreated NIH3T3 cells using phospho-ERK1/2 antibody at 1:500 (upper) and a non-phospho-specific ERK1/2 antibody at 1:500 (lower) .