Neuron Specific Enolase Antibody

Neuron Specific Enolase (NSE, Enolase-2) has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, ELISA: suitable

UniProt

P09104

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to part of the human protein was used as the immunogen for the Neuron Specific Enolase antibody.

Clonality

Monoclonal

Isotype

LgG1

Clone

1G7-B4

Applications

Purity

Protein G affinity

Format

Purified

Buffer

Antibody in 1X PBS with 0.03% ProClin 300

Reconstitution

Aliquot the Neuron Specific Enolase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Neuron Specific Enolase antibody is available for research use only.

Storage Conditions

Aliquot the Neuron Specific Enolase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS with 0.03% ProClin 300

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Neuron Specific Enolase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human 1) SHSY-5Y and 2) U87-MG cell lysates using Neuron Specific Enolase antibody at 1:1000. Predicted molecular weight ~47 kDa.