EpCAM Antibody

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunoprecipitation: suitable

UniProt

P16422

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein was used as the immunogen for this EpCAM antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

1D5-B6-B10

Applications

WB, IP

Purity

Protein G affinity

Format

Purified

Buffer

Antibody in PBS with 50% Glycerol and 0.03% ProClin 300

Reconstitution

Store the EpCAM antibody at -20oC.

Limitations

This EpCAM antibody is available for research use only.

Storage Conditions

Store the EpCAM antibody at -20°C.

Formulation

In PBS with 50% glycerol and 0.03% ProClin 300

Applications Notes

The stated application concentrations are suggested starting points. Titration of the EpCAM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of lysate from CHO-K1, EpCAM transfected CHO-K1, MCF7 (+ control), HCT116 (+ control), and HeLa (- control) cell lysate using EpCAM antibody at 1:1000. Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated) .