APC11 Antibody / ANAPC11

Anaphase-promoting complex subunit 11, together with the cullin protein ANAPC2, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May recruit the E2 ubiquitin-conjugating enzymes to the complex.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000-2000

UniProt

Q9NYG5

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 56-94 from the human protein was used as the immunogen for the APC11 antibody.

Clonality

Polyclonal

Isotype

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the APC11 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This APC11 antibody is available for research use only.

Storage Conditions

Aliquot the APC11 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the APC11 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Mouse

Image Legend

Western blot testing of human 1) HeLa, 2) PC-3, 3) T47D and 4) U-2OS cell lysate with APC11 antibody at 1:2000. Predicted molecular weight: ~10 kDa (Q9NYG5-1) or ~20 kDa (Q9NYG5-2) .