Factor VII Antibody / F7

Coagulation factor VII (F7) initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. F7 is converted to F7a by factor Xa, XIIa, IXa, or thrombin by minor proteolysis. In the presence of tissue factor (TF) and calcium ions, F7a then converts F10 to F10a by limited proteolysis. F7a will also convert F9 to F9a in the presence of TF and calcium.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000-2000

UniProt

P08709

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 302-332 from the human protein was used as the immunogen for the Factor VII antibody.

Clonality

Polyclonal

Isotype

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Factor VII antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Factor VII antibody is available for research use only.

Storage Conditions

Aliquot the Factor VII antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Factor VII antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human NCI-H1299 cell lysate with Factor VII antibody at 1:2000. Predicted molecular weigh ~51 kDa but may be observed at higher molecular weights due to glycosylation.