LARGE Antibody
Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine- beta-4- (phosphate-6-) mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) . Phosphorylated O- mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity and plays a key role in skeletal muscle function and regeneration. LARGE elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure initiated by B3GNT1/B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000-2000
Gene ID
9215
UniProt
O95461
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 365-398 from the human protein was used as the immunogen for the LARGE antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the LARGE antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This LARGE antibody is available for research use only.
Storage Conditions
Aliquot the LARGE antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the LARGE antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Chicken, Mouse
Image Legend
Western blot testing of Jurkat cell lysate with LARGE antibody at 1:2000. Predicted molecular weight: 88 kDa.
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