RSPO1 Antibody / R-spondin-1
R-spondin-1 is an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000-2000
UniProt
Q2MKA7
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 206-240 from the human protein was used as the immunogen for the RSPO1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the RSPO1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This RSPO1 antibody is available for research use only.
Storage Conditions
Aliquot the RSPO1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the RSPO1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of human 1) HL-60, 2) Jurkat and 3) K562 cell lysate with RSPO1 antibody at 1:2000. Predicted molecular weight: 29 kDa.
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