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ROBO1 Antibody

Roundabout homolog 1 is the receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000-1:2000

Gene ID

6091

UniProt

Q9Y6N7

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1097-1130 from the human protein was used as the immunogen for the ROBO1 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the ROBO1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ROBO1 antibody is available for research use only.

Storage Conditions

Aliquot the ROBO1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the ROBO1 antibody may be required due to differences in protocols and substrate sensitivity.

Image Legend

Western blot testing of human 1) HeLa and 2) SH-SY5Y cell lysate with ROBO1 antibody at 1:2000. Expected molecular weight: ~181/250 kDa (unmodified/glycosylated) .

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