CTCFL Antibody
Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR) . Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity) . Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000-2000
Gene ID
140690
UniProt
Q8NI51
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 617-650 from the human protein was used as the immunogen for the CTCFL antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the CTCFL antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CTCFL antibody is available for research use only.
Storage Conditions
Aliquot the CTCFL antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the CTCFL antibody may be required due to differences in protocols and substrate sensitivity.
Image Legend
Western blot testing of human Jurkat cell lysate with CTCFL antibody at 1:2000. Predicted molecular weight: 76 kDa.
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