FZR1 Antibody
Fizzy related protein 1 is a key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000-2000
UniProt
Q9UM11
Host
Mouse
Reactivity
Human
Immunogen
Human recombinant protein corresponding to amino acids 1-496 was used as the immunogen for this FZR1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
1621CT637.76.67
Applications
WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the FZR1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This FZR1 antibody is available for research use only.
Storage Conditions
Aliquot the FZR1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the FZR1 antibody may be required due to differences in protocols and substrate sensitivity.
Image Legend
Western blot testing of human 1) 293T/17 and 2) HeLa cell lysate with FZR antibody at 1:2000. Predicted molecular weight: 55 kDa.
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