Anti-GFP Antibody

Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with asecondary reagent (such as dyes or antibodies) for detection.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:100-1:500

Host

Mouse

Immunogen

Purified His-tagged protein was used to produced this anti-GFP antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

168AT1211.269.64

Applications

WB, ELISA

Purity

Ascites

Format

Ascites

Buffer

In ascites with 0.09% sodium azide

Reconstitution

Aliquot the anti-GFP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This anti-GFP antibody is available for research use only.

Storage Conditions

Aliquot the anti-GFP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In ascites with 0.09% sodium azide

Applications Notes

Titration of the anti-GFP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of anti-GFP antibody using purified GFP/YFP/BFP proteins expressed in bacteria: Both GFP (Lanes 1-3) and YFP (Lanes 4-6) but not BFP (data not shown) were detected.