CD138 Antibody / Syndecan-1

CD138 is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The Syndecan-1/CD138 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:8000, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50

UniProt

P18827

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 210-238 from the human protein was used as the immunogen for this CD138 antibody.

Clonality

Monoclonal

Isotype

IgM

Clone

587CT7.3.6.5

Applications

IF, WB, FACS, ELISA

Purity

Ascites

Format

Ascites

Buffer

In ascites with 0.09% sodium azide

Reconstitution

Aliquot the CD138 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD138 antibody is available for research use only.

Storage Conditions

Aliquot the CD138 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In ascites with 0.09% sodium azide

Applications Notes

Titration of the CD138 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

CD138 antibody flow cytometric analysis of U266 cells (right histogram) compared to a negative control (left histogram) . Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary Ab was used for the analysis