Anti-Oct4 Antibody
This gene encodes POU5F1/Oct-4/OCT4, a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t (6;22) (p21; q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:4000
UniProt
Q01860
Host
Mouse
Reactivity
Human, Mouse
Immunogen
This anti-Oct4 antibody is generated from mouse immunized with POU5F1/OCT4 recombinant protein.
Clonality
Monoclonal
Isotype
IgM
Clone
341CT7.6.2
Applications
WB, ELISA
Purity
Ascites
Format
Ascites
Buffer
In ascites with 0.09% sodium azide
Reconstitution
Aliquot the anti-Oct4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This anti-Oct4 antibody is available for research use only.
Storage Conditions
Aliquot the anti-Oct4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In ascites with 0.09% sodium azide
Applications Notes
Titration of the anti-Oct4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Anti-Oct4 antibody western blot analysis in K562 lysate. Predicted molecular weight ~38/30kDa (isoform A/B), commonly observed at 38-45 kDa.
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