EIF4E Antibody
EIF4E recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Flow Cytometry: 1:10-1:50
UniProt
P06730
Host
Mouse
Reactivity
Human, Mouse
Immunogen
This EIF4E antibody is generated from mouse immunized with EIF4E recombinant protein.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
163CT48.1.9
Applications
WB, FACS, ELISA
Purity
Ascites
Format
Ascites
Buffer
In ascites with 0.09% sodium azide
Reconstitution
Aliquot the EIF4E antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This EIF4E antibody is available for research use only.
Storage Conditions
Aliquot the EIF4E antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In ascites with 0.09% sodium azide
Applications Notes
Titration of the EIF4E antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
EIF4E antibody western blot analysis in Jurkat lysate. Predicted molecular weight ~27 kDa.
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