NGFR Antibody

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:100, Western blot: 1:500-1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

P08138

Host

Mouse

Reactivity

Human, Mouse

Immunogen

This NGFR antibody was produced from mouse immunized with NGFR recombinant protein.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

8G3G10

Applications

IF, WB, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the NGFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This NGFR antibody is available for research use only.

Storage Conditions

Aliquot the NGFR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the NGFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Fluorescent confocal image of SY5Y cells stained with NGFR antibody. Alexa Fluor 488 secondary Ab (green) was used (1:1000, 1h) . Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min) .