Integrin alpha 3 Antibody

Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. Alpha- 3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:2000-4000

UniProt

P26006

Host

Rabbit

Reactivity

Human

Immunogen

This Integrin alpha 3 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 165-199 amino acids from the N-terminal region of human ITGA3.

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Integrin alpha 3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Integrin alpha 3 antibody is available for research use only.

Storage Conditions

Aliquot the Integrin alpha 3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Integrin alpha 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Integrin alpha 3 antibody at 1:4000 dilution and 1) A431 lysate, 2) HeLa lysate, and 3) PC3 lysate at 1:2000. Expected molecular weight: 119-150 kDa depending on glycosylation level.