Pou5f1 Antibody

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') . Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000-2000

UniProt

P20263

Host

Rabbit

Reactivity

Mouse

Immunogen

This mouse Pou5f1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 86-119 amino acids from the N-terminal region of mouse Pou5f1.

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Pou5f1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Pou5f1 antibody is available for research use only.

Storage Conditions

Aliquot the Pou5f1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Pou5f1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Pou5f1 antibody at 1:2000 dilution + F9 lysate; Predicted molecular weight ~38/30kDa (isoform A/B), commonly observed at 38-45 kDa.