Shh Antibody / Sonic Hedgehog

Sonic hedgehog intercellular signaling is essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000-2000

UniProt

Q62226

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

This mouse Shh antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 58-91 amino acids from the N-terminal region of mouse Sonic hedgehog.

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Shh antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Shh antibody is available for research use only.

Storage Conditions

Aliquot the Shh antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Shh antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The 45 kDa precursor protein autocleaves into 27 kDa amino and 19 kDa carboxy fragments.