Kdm6a Antibody

Histone demethylase that specifically demethylates 'Lys- 27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Demethylation of 'Lys-27' of histone H3 is concomitant with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A (By similarity) .

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:2000

UniProt

O70546

Host

Rabbit

Reactivity

Mouse

Immunogen

This mouse Kdm6a antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 1041-1074 amino acids from the C-terminal region of mouse Kdm6a.

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Kdm6a antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Kdm6a antibody is available for research use only.

Storage Conditions

Aliquot the Kdm6a antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Kdm6a antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Kdm6a antibody at 1:2000 dilution. Lane 1: mouse cerebellum lysate; 2: mouse spleen lysate; Predicted molecular weight ~ 154 kDa.