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Nkx2.5 Antibody

Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B. Together with PBX1, it is required for spleen development through a mechanism that involves CDKN2B repression.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000-2000

UniProt

P42582

Host

Rabbit

Reactivity

Mouse

Immunogen

This mouse Nkx2.5 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 98-133 amino acids.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Nkx2.5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Nkx2.5 antibody is available for research use only.

Storage Conditions

Aliquot the Nkx2.5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Nkx2.5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Nkx2.5 antibody at 1:2000 dilution and mouse tissue; Lane 1: stomach lysate; 2: spleen lysate; 3: heart lysate; Predicted molecular weight ~35kDa, routinely observed at 35~45kDa.
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