DKK1 Antibody

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000-1:4000

UniProt

O94907

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This DKK1 antibody was produced from a rabbit immunized with a recombinant protein of human DKK1.

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the DKK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This DKK1 antibody is available for research use only.

Storage Conditions

Aliquot the DKK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the DKK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of lysate from (1) A549, (2) HeLa, (3) HepG2, (4) Jurkat, (5) mouse NIH3T3 cell line using DKK1 antibody. Predicted molecular weight: 26-40 kDa depending on glycosylation level.