Dnmt1 Antibody
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. Three families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1000
UniProt
P13864
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 881-914 from the mouse protein was used as the immungen for the Dnmt1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Dnmt1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Dnmt1 antibody is available for research use only.
Storage Conditions
Aliquot the Dnmt1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the Dnmt1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of lysate from human K562 and mouse F9 cell line using Dnmt1 antibody at 1:1000. Predicted molecular weight: 180-200 kDa
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