POU5F1 Antibody

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') . Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

Q01860

Host

Rabbit

Reactivity

Human

Immunogen

This POU5F1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 123-157 amino acids from the Central region of human POU5F1.

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the POU5F1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This POU5F1 antibody is available for research use only.

Storage Conditions

Aliquot the POU5F1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the POU5F1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of lysate from NCCIT cell line using POU5F1 antibody at 1:1000. Predicted molecular weight ~38/30kDa (isoform A/B), commonly observed at 38-45 kDa.